Dados do Trabalho

Título

EFFECT OF BLUEBERRY EXTRACTS OBTAINED USING NADES OR CONVENTIONAL ORGANIC SOLVENTS IN PARAMETERS AFFECTED BY AN INTESTINAL DYSBIOSIS MODEL IN RATS

Introdução

Blueberries (Vaccinium sp.) are fruits rich in phenolic compounds (PC), which have promising health benefits. Natural Deep Eutectic Solvents (NADES) are ecofriendly and biocompatible solvents that have been shown to increase the bioavailability of PC. PC have been pointed as prebiotic compounds suggesting their putative usefulness for reducing intestinal dysbiosis. This study aimed to assess the effects of a blueberry crude extract obtained using NADES (CE-NADES) or a conventional organic solvent (CE-SORG) in intestinal dysbiosis induced by an antibiotic cocktail (AB) in rats (CEUA: 3015110219-UFSM).

Material e Métodos

Adult wistar rats were divided into control (n=32) and dysbiosis (n=32) groups that received water or AB by gavage (2 mL/kg b.w.) for 10 days. Both groups were further divided into four subgroups (control, NADES, CE-NADES, and CE-SORG, n=8/group). Blueberry extracts were administered at 10 mg of PC/kg b.w. for 11 days, beginning concurrently with the administration of the AB cocktail. On day 12, rats were euthanized and tissues and feces were collected for subsequent analyses.

Resultados e Discussão

The dysbiosis group had increased cecum weight (16.0 ± 1.2 g versus 6.6 ± 0.6 g, p<0.05) and reduced fecal water content (14.3 ± 0.5% versus 21.1 ± 1.1%, p<0.05) compared to the control group. AB reduced short chain fatty acids (SCFA) levels (mmol/kg of feces) compared to the control group (p<0.05): acetic acid (28.5 ± 4.0 vs 72.3 ± 16.0), propionic acid (1. 1 ± 0.2 vs 14.9 ± 2.9), and butyric acid (not detected vs 11.2 ± 3.0). Changes in SCFA caused by dysbiosis were not prevented by blueberry extracts (p>0.05).

Conclusão

The concurrent administration of CE-NADES or CE-SORG and AB cocktail did not prevent the physiological dysfunctions assessed in the present study. Our research continues to explore other effects of blueberry extracts in the studied model of intestinal dysbiosis.