Dados do Trabalho

Título

CHICKEN CELL ADHESION IN PLANT-BASED MICROCARRIERS AND SCAFFOLDS

Introdução

The process of obtaining primary cells commences with a small sample of animal cells typically acquired via biopsy. These cells are subsequently cultured within a nutrient-rich growth medium, facilitating their multiplication. Subsequently, depending on the desired end-product, the cells are either seeded onto scaffolds or microcarriers.

Material e Métodos

Primary chicken myoblast cells were isolated from 15-day-old specific-pathogen-free (SPF) White Leghorn chicken embryos and cultivated in tissue culture flasks with Dulbecco’s modified Eagle’s medium (DMEM-High Glucose) supplemented with suitable growth factors and antibiotics until they reached confluent monolayers. Commercial scaffolds and microcarriers from different plant-based origins (potato, soy, gelatin, chitosan, lupin, HybridX, HybridX Fluff, and their combinations) were previously processed following the manufacturer's recommendations. Cells were trypsinized, labeled with carboxyfluorescein succinimidyl ester (CFSE) and seeded on the scaffolds and microcarriers. Cell morphology was assessed along the time (after 2 h of cell seeding and after 24 h of incubation) on different substrates by microscopy.

Resultados e Discussão

Among the evaluated microcarriers, HybridX showed greater amounts of protein (13%) than the potato microcarrier after chicken cell growth. The HybridX microcarrier exhibited enhanced cell adhesion and proliferation, likely attributed to the inclusion of gelatin in its composition, which facilitates cell attachment. Regarding the scaffolds, the chitosan-lupin combination showed the most promising attachment to chicken cells and, consequently, the highest protein content (10%), followed by the HybridX Fluff (8.9%).

Conclusão

This study explores the efficacy of various plant-based scaffolds and microcarriers for the cultivation of primary chicken myoblast cells, crucial for the development of cultured meat. By isolating cells from SPF White Leghorn chicken embryos and employing different substrates, the research sheds light on optimal conditions for cell attachment and protein production. The findings not only contribute to advancing the field of cultured meat production but also highlight the potential of sustainable materials in tissue engineering applications.