Dados do Trabalho

Título

EFFECTS OF REPEATED AND COMBINED TREATMENT OF ARIPIPRAZOLE AND TOPIRAMATE ON THE METHYLPHENIDATE-INDUCED CONDITIONED PLACE PREFERENCE IN MICE

Fundamentação/Introdução

Methylphenidate (MET) is a psychostimulant characterized by blocking dopamine reuptake in the synaptic cleft, which is capable of producing dependence behaviors, but effective pharmacological therapies for dependence are still limited.

Objetivos

The present study aimed to evaluate the rewarding effects of different doses of MET on the paradigm of conditioned place preference (CPP) and subsequently the effects of repeated and combined treatment of aripiprazole (ARP) and topiramate (TOP) on the MET-induced CPP on 3-month-old female Swiss mice (CEUA-UESC nº 05/2016).

Delineamento e Métodos

In the experiment 1 (EXP 1) the animals were submitted to a preconditioning test followed by conditioning with MET (1.8, 3.0, 5.7, and 10.0 mg/kg) on odd days and saline (SAL) on even days during 8 daily sessions. On the 10th day the CPP was quantified. The results showed MET-induced CPP on two higher doses (p<0.05). The 10 mg/kg dose was used to induce CPP in the following experiments. Thus, after post-conditioning test, the animals were treated with SAL in the CPP compartment previously associated with saline an odd days, or vehicle (VEI) or TOP (2.5, 5.0 and 10.0 mg/kg – EXP 2) or ARP (0.025, 0.05, 0.075, and 0.1 mg/kg – EXP 3) or TOP+ARP (5.0 and 0.025 mg/kg – EXP 4) in the CPP compartment previously associated to MET on odd days for 8 daily sessions. They were reexposed to MET and 24 hours after CPP was quantified.

Resultados

The results showed that individual treatment with TOP2.5 (265.8±18.92), ARP0.075 (277.1±42.40) and ARP0.1 (289.0±26.47) attenuated the CPP when compared to VEI (EXP2 371.9±16.29; EXP3 407.6±11.98, respectively). The combination of non-effective doses of TOP5.0 + ARP0.025 (292.7±16.93) also prevented the expression of CPP when compared to VEI (369.3±24.40) [Two-way ANOVA/Bonferroni post hoc - p <0.05].

Conclusões/Considerações Finais

Our results suggest that both reduces the rewarding properties of MET and corroborate other studies indicating them as good candidates for addiction treatment. This summary was presented at SBNeC 2018.