Dados do Trabalho

Título

EFFECTS OF REPEATED METHYLPHENIDATE TREATMENT IN MICE´S CHILDHOOD ON ANXIETY IN THEIR ADULT LIFE.

Fundamentação/Introdução

INTRODUCTION: Methylphenidate (MET) has mechanisms of action similar to amphetamines, it also has a potential for significant behavioral changes after its use. Some of the concerns in the use of methylphenidate are related to its inappropriate use and the
development of side effects to the chronic use of this drug after exposure during childhood.

Objetivos

OBJECTIVES: Considering all above, this work aimed to verify the effects of chronic treatment of MET during mice`s childhood of on anxiety.

Delineamento e Métodos

METHODS: For this purpose, 32 Swiss females with 30 days of life were used. They were housed in groups of 8 with light and dark cycle of 12 hours, water and food ad libidum. The animals received an intraperitoneal injection(I.P.) of MET (MET) or vehicle (VEI) and
set back to their house cage from 30th to the 39th day of life. They remained without any intervention until their 69th day of life.
After this period (Day 70) they were submitted to the Plus Maze Avoidance Task (AT) protocol for anxiety test. In order to
evaluate anxiety levels, the following parameters were evaluated during the AT training: open arm time% (OAT), time
exploration at the end of open arms (TE-EOA), head dipping (HD), these last two parameters as indicators of fear/anxiety like
behavior. This study was approved by the local ethics committee (Comitê de Ética em Pesquisa da Universidade Estadual de
Santa Cruz) at protocol number 009/17.

Resultados

RESULTS: MET group had a lower percentage of OAT (25.81 ± 3.04), TE-EOA (0.125 ± 0.08539) and HD (4.188 ± 1.058) when compared
to the VEI group with OAT (36.86 ± 3.07), TE-EOA (1.25 ± 0.4787) and HD (9.75 ± 1.545) [unpaired t-test - p < 0,05].

Conclusões/Considerações Finais

CONCLUSIONS: The results suggest the development of anxiogenic behavior in the animals that received MET in childhood. This summary was presented at SBNeC 2018.

Referências

SILVA, R.H.; FRUSSA-FILHO, R. The plus-maze discriminative avoidance task: a new model to study memory-anxiety interactions. Effects of chlordiazepoxide and caffeine. J Neurosci Methods. 2000 Oct 30;102(2):117-25.

Palavras-chave

Methylphenidate, Anxiety, Plus-maze Discriminative Avoidance Task